Aptitude

Publications

aptitude is revolutionizing molecular recognition

Discovery

J.P. Wang, J. Yu, Q. Yang, J. McDermott, A. Scott, M. Vukovich, R. Lagrois, Q. Gong, W. Greenleaf, M. Eisenstein, B. S. Ferguson, H. T. Soh
Multi-Parameter Particle Display (MPPD): A Quantitative Screening Method for Discovery of Highly Specific Aptamers
Angewandte Chemie International Edition, DOI: 10.1002/anie.201608880 (2016)

H. Qu, A.T. Csordas, J.P. Wang, S-S Oh, M. Eisenstein, and H.T. Soh
Rapid and label-free strategy to select aptamers for metal ions
ACS Nano 10 (8) 7558-7565 (2016)

A. Csordas, A. Jorgensen, J.P. Wang, E. Gruber, Q. Gong, E. Bagley, M. Nakamoto, M. Eisenstein, and H.T. Soh
High-throughput discovery of aptamers for sandwich assays
Anal. Chem., 88 (22), pp 10842–10847 (2016)

J.P. Wang, Q. Gong, N. Maheshwari, M. Eisenstein, M.L. Arcila, K.S. Kosik, H.T. Soh
Particle Display: A Quantitative Screening Method for Generating High-Affinity Aptamers
Angew. Chemie. Int'l Ed. 126 (19) 4896–4901 (2014)

K. Ahmad, Y. Xiao and H.T. Soh
Selection is More Intelligent than Design: Improving the Affinity of a Bivalent Ligand through Directed Evolution
Nucleic Acids Research 40.22 11777-11783 (2012)

J. Wang, J.F. Rudzinski, Q. Gong, H. T. Soh, P.J. Atzberger
Influence of Target Concentration and Background Binding on In Vitro Selection of Affinity Reagents,
PLoS One Vol. 7, Issue 8, e43940 (2012)

Q. Gong, J. Wang, K. M. Ahmad, A. T. Csordas, J. Zhou, J. Nie, R. Stewart, J. Thomson, J. Rossi, and H. T. Soh
Selection Strategy to Generate Aptamer Pairs that Bind to Distinct Sites on Protein Target,
Analytical Chemistry Vol. 84 5365−5371 (2012)

J. Wang, Y. Liu, T. Teesalu, K. N. Sugahara, V. R.Kotamraju, J.D. Adams, B.S. Ferguson, Q.Gong, S. S. Oh, A.T. Csordas, M. Cho, E. Ruoslahti, Y. Xiao, and H. T. Soh
Selection of Phage-Displayed Peptides on Live Adherent Cells in Microfluidic Channels
Proceedings of the National Academy of Sciences, USA, Vol. 108, No. 17 (2011)

X.H. Lou, J. Qian, Y. Xiao, L. Viel, A. E. Gerdon, E. T. Lagally, P. Atzberger, T. M.Tarasow, A. J. Heeger, and H. T. Soh
Micromagnetic Selection of Aptamers in Microfluidic Channels
Proceedings of the National Academy of Sciences, USA, Vol. 106, No. 9, 2989-2994 (2009)

 

Characterization and Optimization

S. Meyer, J. P. Maufort, J. Nie, R. Stewart, B. McIntosh, L. Conti, K. M. Ahmad, H. T. Soh and J.A. Thomson
Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents
PLoS One Vol. 8, Issue 8 (2013)

M. Cho, S. S. Oh, J. Nie, R. Stewart, M. S. Eisenstein, J. Chambers, J. D. Marth, F. Walker, J. A. Thomson, H. T. Soh
Quantitative Selection and Parallel Characterization of Aptamers
Proceedings of the National Academy of Sciences, USA (in press, 2013)

M. Cho, Y. Xiao, J. Nie, R. Stewart, A. Csordas, S. S. Oh, J. Thomson, H. T. Soh
Quantitative Selection of DNA Aptamers through Microfluidic Selection and High Throughput Sequencing
Proceedings of the National Academy of Sciences, USA, Vol. 107, No. 35, 15373-15378 (2010)

 

Application

P. L. Mage, B. S. Ferguson, D. Maliniak, K. L Ploense, T. E. Kippin and H. T. Soh
Closed-loop control of circulating drug levels in live animals
Nature Biomedical Engineering 1, 0070 (2017)

B.S. Ferguson, D.A. Hoggarth, D. Maliniak, K. Ploense, R.J. White, N. Woodward, K. Hsieh, A.J. Bonham, M. Eisenstein, T. Kippin, K.W. Plaxco and H.T. Soh
Real-time, aptamer-based tracking of circulating therapeutic agents in living animals
Science Translational Medicine 5, 213ra165 (2013)

S.S. Oh, K. Plaxco, Y. Xiao, M. Eisenstein, and H.T. Soh
In Vitro Selection of Shape-Changing DNA Nanostructures Capable of Binding-Induced Cargo Release
ACS Nano 7 (11) 9675–9683 (2013)

I. Olmsted, Y. Xiao, M. Cho, A. Csordas, J. Sheehan, J. Meiler, H. T. Soh, and D. Bornhop
Measurement of Aptamer-Protein Interactions with Back-Scattering Interferometry
Analytical Chemistry Vol 83. 8867-8870 (2011)

B. S. Ferguson, S. F. Buchsbaum, T.-T. Wu, K. Hsieh, Y. Xiao, R. Su,n and H. T. Soh
Genetic Analysis of H1N1 Influenza Virus from Throat Swab Samples in a Microfluidic System for Point-of-Care Diagnostics
Journal of the American Chemical Society 133, 9129-9135 (2011)

A. Csordas, A. E. Gerdon, J. D. Adams, J. Qian, S.S. Oh, Y. Xiao, and H. T. Soh
Detection of Proteins in Serum via Micromagnetic Aptamer PCR (MAP) Technology
Angewandte Chemie International Edition (49) 355-358 (2010)

B.S. Ferguson, S. Buchsbaum, J. Swensen, K. Hsieh, X.H. Lou, and H.T. Soh
Integrated Microfluidic Electrochemical DNA Sensor
Analytical Chemistry Vol. (81) 6503-6508 (2009)

J. Swensen, Y. Xiao, B.S. Ferguson, A. Lubin, R. Lai, A.J Heeger, K.W. Plaxco, and H.T. Soh
Continuous Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic Electrochemical
Aptamer-Based Sensor Journal of the American Chemical Society (131) 4262-4266 (2009)